By Kim Rogers, Ashok Mulchandani
A state of the art selection of targeted, step by step thoughts and protocols for developing, comparing, and utilizing affinity-based biosensors. perfect for newbies beginning study of their box or skilled researchers desirous to use a biosensor for a particular analytical dimension, the tools distinct right here enable biochemists, analytical chemists, microbiologists, and engineers to effectively observe biosensor know-how to their particular difficulties. The concepts contain using antibodies and membrane receptors to build optical, thermal, acoustic, and electrochemically dependent biosensors. extra innovations contain antibodies, receptors, nucleic acids, liposomes, and eukaryotic cells. A spouse quantity, Enzyme and Microbial Biosensors: Protocols and strategies, through Mulchandani & Rogers, concentrates on enzyme-biosensors.
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Additional info for Affinity Biosensors: Techniques and Protocols (Methods in Biotechnology)
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Two different strategies were considered for the munobihzation of the rhGM-CSF molecule: (1) direct immobilization of rhGM-CSF in the matrix using amine coupling; and (2) immobilization of a capturing antibody that is used to bind the first MAb to be tested for. The rhGM-CSF molecule is then bound to this captured MAb. The first method turned out to give very irreproducible readings. This IS most likely as a result of blocking of one or several epitopes during the immobilization procedure. A few of the NH2 groups used in the amine-coupling sequence are probably located near the epitope(s), which then become sterically deactivated and thereby unable to bmd to the subsequently injected MAbs.
Affinity Biosensors: Techniques and Protocols (Methods in Biotechnology) by Kim Rogers, Ashok Mulchandani