By Mei-Ling Ting Lee
After genomic sequencing, microarray know-how has emerged as a universal platform for genomic reports within the existence sciences. Microarray know-how offers a scientific solution to survey DNA and RNA edition. With the abundance of knowledge made from microarray reviews, despite the fact that, the last word effect of the experiences on biology will rely seriously on facts mining and statistical research. The contribution of this booklet is to supply readers with an built-in presentation of varied themes on reading microarray data.
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Extra resources for Analysis of Microarray Gene Expression Data
There is no general consensus on the usage of the terms probe and target, and researchers have used these two terms interchangeably in a number of publications. With respect to the nucleic acids whose entwining represents the hybridization reaction, the identity of one is defined as it is tethered to the solid phase, making up the microarray itself. The identity of the other is revealed by hybridization. 15 adopted the nomenclature that the tethered nucleic acids spotted on the array are the probes, and the fluor-tagged cDNAs from a complex mRNA mixture extracted from cells are the targets.
There are several software packages, both commercial and freeware, available for image analysis and quantification of spotted microarrays. The formats of the result files vary greatly. Typically, spreadsheets are used to report raw array data, including the location of the spots, gene identity, mean and median of the pixel intensities within a spot, and local background intensities. The simultaneous hybridization of two specimen samples labeled with Cy3 (green) and Cy5 (red) dyes has special analysis requirements.
Two methods of choice are Northern blot analysis and quantitative real-time PCR. 2. Northern Blot Analysis Specific RNA sequences can be detected by blotting and hybridization analysis using techniques very similar to those originally developed by Ed Southern for DNA. Different RNA molecules can be size-separated or fractionated by gel electrophoresis. The gel electrophoresis is run un der denaturing conditions to keep the single-stranded RNA molecules denatured, thus allowing good separation.
Analysis of Microarray Gene Expression Data by Mei-Ling Ting Lee